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| Registros recuperados: 47 | |
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| Auffret, Mathieu; Diouris, M; Herry, A. |
| A polyclonal rabbit anti-human lysozyme (Dako) was applied on histological sections from bivalve mollusc and revealed with a mouse anti-rabbit IgG fluorescein conjugated antibody to localise cellular lysozyme in tissues where its activity had previously detected by enzymatic techniques. Labelling occurred in the cytoplasm of epithelial cells covering the body. This is in agreement with the intracellular distribution of this enzyme. In the Lucinid Lucinella divaricata gills, the cytoplasm of bacteriocytes was also labelled. This suggests that antibacterial activity occurred in these cells. Control sections indicate that fluorescence was a consequence of true primary antibody fixation. However, its specificity should be verified by electrophoretical... |
| Tipo: Text |
Palavras-chave: Bivalvia; Immunology; Antibodies; Electrophoresis; Histology. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-1723.pdf |
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| BARRAVIERA,B.; SARTORI,A.; PEREIRA DA SILVA,M. F.; KANENO,R.; PERAÇOLI,M. T. S.. |
| A sandwich-type ELISA technique for specific and sensitive detection of Crotalus durissus terrificus venom antigens, horse-antivenom, human IgG and IgM antibodies was set up. Sixteen patients, 13 males and 3 females aged between 13 to 63 years (mean 33 ± 15) bitten by Crotalus durissus terrificus snakes were studied. Of the 15 patients, 6 had previously received anti-Crotalus venom and no seric venom was detected. For the other 9 patients studied, the venom levels ranged from 2 to 108 ng/ml according to the severity of each case. Seric antivenom was detected up to 44 days after the bite. IgM human antibody levels against Crotalus venom were higher between 3 and 18 days after specific treatment. IgG human antibody levels against Crotalus venom were detected... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzyme-linked immunosorbent assay; Crotalus durissus terrificus; Venom; Antivenom; Antibodies; Cerebrospinal fluid. |
| Ano: 1996 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301996000100003 |
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| BERGDOLL,M. S.. |
| Toxic shock syndrome is a staphylococcal disease caused by toxins produced by the staphylococci, toxic shock syndrome toxin-1 and enterotoxin B. The disease results from staphylococci growing in the vagina with the use of tampons during menstruation, primarily in young women. However, any staphylococcal infection can result in toxic shock syndrome if the staphylococci produce the appropriate toxins and the individual has no antibodies to the toxins. The symptoms can be quite severe, with high fever, low blood pressure, diffuse macular erythroderma, orthostatic dizziness, vomiting and or diarrhea at the onset, severe myalgia, peeling of the skin of the palms of the hands and the soles of the feet after seven to ten days, and death in some cases. The disease... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxic shock syndrome; Toxic shock syndrome toxin; Staphylococci; Enterotoxins; Antibodies. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301997000100002 |
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| Veloso,D.. |
| Low and high molecular weight kininogens (LK and HK), containing 409 and 626 amino acids with masses of ~65 and 120 kDa after glycosylation, respectively, are coded by a single gene mapped to the human chromosome 3 by alternative splicing of the transcribed mRNA. The NH2-termini Glu1-Thr383 region, identical in LK and HK, contains bradykinin (BK) moieties Arg363-Arg371. LK, HK and their kinin products Lys-BK and BK are involved in several biologic processes. They are evolutionarily conserved and only 7 patients, all apparently normal, have been reported to lack them. In one of these patients (Williams' trait), a codon mutation (Arg178 <FONT FACE="Symbol">®</FONT> stop) has been blamed for the absence of LK and HK. However, using Western blots... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Kininogen deficiency; Kinins; Antibodies; Plasma; Kininogen-like species; Human and nonhuman primates. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700004 |
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| Passos,L.M.F.; Ribeiro,M.F.B.; Anderegg,P.I.; Böse,R.. |
| O objetivo do presente estudo foi determinar a dinâmica da produção de anticorpos anti-Babesia equi e B. caballi em éguas gestantes em condições naturais de campo em Minas Gerais e avaliar a eficiência da transferência passiva de anticorpos via colostro. Foram analisadas, pela imunofluorescência indireta, amostras de soros colhidas de 34 fêmeas aos 290, 305 e 320 dias de gestação, e no momento do parto, e de seus respectivos potros até 36 horas após o nascimento. Aos 290 dias de gestação, todas as fêmeas estavam positivas para B. equi e apenas duas estavam negativas para B. caballi. Todos os potros tornaram-se positivos para B. equi após ingestão de colostro, entretanto 42% permaneceram negativos para B. caballi 36 horas após o nascimento. Não se observou... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Babesia equi; Babesia caballi; Pregnant mares; Antibodies. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000600003 |
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| Donnelly,J.J.; Ulmer,J.B.. |
| DNA plasmids encoding foreign proteins may be used as immunogens by direct intramuscular injection alone, or with various adjuvants and excipients, or by delivery of DNA-coated gold particles to the epidermis through biolistic immunization. Antibody, helper T lymphocyte, and cytotoxic T lymphocyte (CTL) responses have been induced in laboratory and domesticated animals by these methods. In a number of animal models, immune responses induced by DNA vaccination have been shown to be protective against challenge with various infectious agents. Immunization by injection of plasmids encoding foreign proteins has been used successfully as a research tool. This review summarizes the types of DNA vaccine vectors in common use, the immune responses and protective... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plasmid; Nucleic acid; Protection; Antibodies; T cells; CTL. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200010 |
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| Costa,P.C.S.; Fortes,F.S.A.; Machado,A.B.; Almeida,N.A.C.; Olivares,E.L.; Cabral,P.R.; Pedrosa,R.C.; Goldenberg,R.C.; Campos-de-Carvalho,A.C.; Masuda,M.O.. |
| We report results obtained with sera from 58 chronic chagasic patients that were evaluated for effects on heart rate and atrioventricular (AV) conduction in isolated rabbit hearts and screened for the presence of muscarinic and beta-adrenergic activity. We show that sera from 26 patients decreased heart rate, while 10 increased it and 22 had no effect. Additionally, sera from 20 of the 58 patients blocked AV conduction. Muscarinic activation seems to be involved in both effects, but is not the only mechanism, since atropine did not antagonize the decrease in heart rate in 23% of sera or AV block in 40%. Sera from patients with complex arrhythmias were significantly more effective in depressing both heart rate and AV conduction. Sera that induce increases... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chagas' disease; Antibodies; Muscarinic activation; Beta-adrenergic activation; Gap junction; Mammalian heart. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400010 |
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| LIPPS,B. V.. |
| Lethal Toxin Neutralizing Factor (N-LTNF), MW 63.0 kDa, was isolated from opossum serum. After trypsin digestion, the active domain of N-LTNF was isolated and sequenced. The synthetic peptide consisting of ten amino acids was designated as LT-10. N-LTNF and LT-10 inhibited the lethality of animal, plant and bacteria toxins when tested on mice non-immunologically. The antibodies against N-LTNF and LT-10 only reacted immunologically with toxins and not with non-toxic substances. Anti-LTNF and anti-LT-10 reacted immunologically by ELISA test with toxins that were not detected by mouse test, such as cholera toxin and digoxin. Anti-LTNF and anti-LT-10 failed to react immunologically with non-toxic substances, such as nerve growth factor and collagen. Currently,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antibodies; In vitro assay; Lethal toxin neutralizing factor; Toxins; Venoms. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200003 |
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| Brandão,Paulo Eduardo; Cortez,Adriana; Ferreira,Fernando; Ferreira Neto,José Soares; Gregori,Fábio; Heinemann,Marcos Bryan; Homem,Valéria Stacchini Ferreira; Rodriguez,Cesar Alejandro Rosales; Jerez,José Antonio. |
| Rotavirus is a worldwide etiologic agent of diarrhea, responsible for large economic losses. We studied the seroprevalence of antibodies to group A rotavirus in cattle in 67 smallholder farms from Uruará municipality, using counterimmunoelectroosmophoresis with the NCDV strain as a standard antigen. Prevalence of positive smallholder farms was 95.6-100%. Significant differences were seen between age groups when the seropositivity rose from the youngest to the oldest groups and between females and males older than 1 year, when the seropositivity was higher in the first group. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Seroepidemiologic studies; Antibodies; Rotavirus; Cattle; Amazonia. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000100003 |
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| Kiffer,Carlos Roberto Veiga; Conceição,Orlando Jorge; Santos,Edgar Bortholi; Sabino,Ester; Focaccia,Roberto. |
| OBJECTIVES: Estimate the prevalence of immunity to poliomyelitis (anti-polio antibodies) in the city of São Paulo/Brazil through a population-based survey. METHODS: A quantitative and inductive method was used to draw a representative sample of the population. Randomization and stratification (based on sex, age and residence region) was done, and 1,059 individuals were studied on a home-visit basis (structured questionnaires and blood samples). A microneutralization test was performed to detect anti-polio antibodies against serotypes 1, 2 and 3. RESULTS: The estimated prevalence of immunity to poliomyelitis was high, with 94.6% prevalence of anti-polio 1 antibodies, 98.8% anti-polio 2 and 91.9% anti-polio 3. Despite this high prevalence, there were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immunity prevalence; Poliomyelitis; Poliviruses; Seroepidemiologic studies; Antibodies; Viral blood. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000500004 |
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| Lima,V.M.F.; Gonçalves,M.E.; Ikeda,F.A.; Luvizotto,M.C.R.; Feitosa,M.M.. |
| Visceral leishmaniasis in Brazil is caused by Leishmania (Leishmania) chagasi and the dog is its most important reservoir. The clinical features in dogs include loss of weight, lymphadenopathy, renal failure, skin lesions, fever, hypergammaglobulinemia, hepatosplenomegaly, anemia, and, rarely, neurological symptoms. Most infected animals develop active disease, characterized by high anti-leishmania antibody titers and depressed lymphoproliferative ability. Antibody production is not primarily important for protection but might be involved in the pathogenesis of tissue lesions. An ELISA test was used to determine if there is an association between neurological symptoms and the presence of anti-L. chagasi antibodies in cerebrospinal fluid (CSF). Thirty serum... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Leishmania (Leishmania) chagasi; Visceral leishmaniasis; Cerebrospinal fluid; Dogs; Antibodies. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400010 |
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| Registros recuperados: 47 | |
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